What is the correct structure of the native protein
Solution-Five step protocol, Using a five step protocol,
August 4, 2017What are main types of enzyme mediated reactions in cells
August 4, 2017A protein has a molecular mass of 200 kDa as determined by gel filtration (=native molecular weight). When separated by SDS-PAGE (without a reducing agent present) only one polypeptide appeared with a mass corresponding to 100 kDa. When determined by SDS-PAGE in combination with a reducing agent (DTT) exactly two polypeptides appeared of sizes 40 and 60 kDa. What is the correct structure of the native protein?
Select one:
a. The native protein is made of one 40 kDA and one 60 kDA polypeptides
b. The native protein is made of two 100 kDa polypeptides
c. The native protein is made of two 40 kDA and two 60 kDA polypeptides
d. The native protein is made of two 100 kDa polypeptides, one 40 kDA and one 60 kDA polypeptides
e. None of the answers is correct.
