Guaiacol concentrationg of the enzyme reaction
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August 4, 2017Of which two proteins do vertebrate hemoglobins consist
August 4, 2017Question: Determine the final guaiacol concentrationg (answers in units of guaiacol) of the following enzyme reaction solutions listed in the table below. Keep in mind that each reaction solution has a total volume of 2000 microliters.
| Tube | 0.05M Buffer | 20mM Hydrogen Peroxide | 80 mM Guaiacol | 0.5 units/ml Peroxidase | Total Volume |
| Tube 1 | 1000microliters | 400 microliters | 350 microliters | 250 microliters | 2000 ul |
| Tube 2 | 800microliters | 400 microliters | 550 microliters | 250 microliters | 2000 ul |
| Tube 3 | 600microliters | 400 microliters | 750 microliters | 250 microliters | 2000 ul |
