Plankton Sampling

Abstract:-
•    Why
•    What
•    How
•    Key findings
(100 – 150 words max.)

Introduction:-
•    Background
•    Objectives
•    Hypothesis
(~ 1 page)

Materials and Methods:
Study site: I will fill out.
Methods used in field:-
Sampling Protocol
To investigate the abundance of both zooplankton and phytoplankton, samples were collected from four sites along the river. Sampling took place on the 6th of September 2011. Sampling was conducted in the morning between the hours of 9.30am-11am. Samples were taken using two different methods from four sites each with three replicates.
Phytoplankton Sampling
Three 250ml bottleswere used for each of the four sites. The bottle was dipped into the river upside down to a certain depth and then flipped back upwards and sealed, each bottle was labelled appropriately and stored to be examined for phytoplankton in the lab.

Zooplankton Sampling
Three 10 Litre buckets were used for each of the four sites. The 10 litre buck was filled with water from the site and then funnelled into a 200µm mesh size, allowing organisms larger than that size to be caught. The resulting sample was then removed by flushing the mesh with distilled water into a sampling jar. The already funnelled water (water without the 200µm organisms) was then funnelled into a 55µm mesh size. The resulting sample was then removed by flushing the mesh with distilled water into a sampling jar. The sampling jars were labelled and stored for later analysis in the lab. In total for each site there were three replicates of 200µm and three replicates of 55µm.
Transect Sampling
Also, the vegetation at each site was examined using a transect. At each site, using a measuring tape, a transect was randomly placed. The line was from measured from the river towards the vegetation. A certain measurement for each site was taken and at each different type of vegetation the vegetation type and measurement was recorded. The overall vegetation percentage was calculated after doing three replicates at each of the four sites.
Laboratory Protocol
Samples were analysed for phytoplankton using a compound microscope with a Sedgewick rafter counting cell. Samples were stirred slightly to ensure a homogenised mix to be sampled, 1ml of sample was then taken and added to the counting cell upon which only living plankton were observed and recorded.
Zoo-plankton was analysed using a dissecting microscope and the sample spread in 1 ml droplets around a petri-dish. The petri-dish was placed on the dissecting microscope and live motile zooplankton were observed and recorded.
Statistical Analysis
(~ 1.5 pages)

Results:-
Statements on findings, Supported by figures and tables, and statistical data analysis
(~ 3 – 4 pages)

Discussion:-
Compare findings with literature
Conclusion
(~ 1 – 2 pages)

References:
Follow format for “Marine and Freshwater research” – Harvard (alphabetical)
Only scientific journals and papers to be used
(~ ½ page)

Format:
– 8 pages including figures, tables and references
– Double – spaced, 12 points Times New Roman (or 10 point Arial)
– A4 with 2.5 cm margins (right and left) and 2 cm margin (top and bottom)
– Include page numbers
– Figures and Tables should be labelled. Numbered and referred to in the text
– References: Single spaced, hanging (0.6 cm)

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