When an agar plate is inoculated, why is the loop sterilized after the initial inoculum is put on? Distinguish between a pure culture and a mixed culture.
Discuss two (2) recommendations the authors make regarding the applicability of performance budgeting to the current United States government. Assume you have been appointed as the new administrator for a federal agency.
August 4, 2017Analyze the implications and challenges of cost, quality, and external forces on electronic health record (EHR) or electronic medical record (EMR) selection and implementation within your department.
August 4, 2017When an agar plate is inoculated, why is the loop sterilized after the initial inoculum is put on? Distinguish between a pure culture and a mixed culture.
Create a 2- to 3-page document in Microsoft Word for providing answers to questions in the following review sheets:
Support your responses with examples.
Cite any sources in APA format.
Exercise 4: Pure bacterial colonies
1. When an agar plate is inoculated, why is the loop sterilized after the initial inoculum is put on?
2. Distinguish between a pure culture and a mixed culture.
3. Define a bacterial colony. List four characteristics by which bacterial colonies may be distinguished.
4.Why should a Petri dish not be left open for any extended period?
5. Why does the streaking method you used to inoculate your plates result in isolated colonies?
Exercise 5: Pour plate and streaking technique to obtain pure cultures
1. Discuss the relative convenience of pour- and streak-plate techniques in culturing clinical specimens.
2. How do you decide which colonies should be picked from a plate culture of a mixed flora?
3. Why is it necessary to make pure subcultures of organisms grown from clinical specimens?
4. What kinds of clinical specimens may yield a mixed flora in bacterial cultures?
5. When more than one colony type appears in pure culture, what are the most likely sources of extraneous contamination?
Exercise 3: Primary media for isolation of microorganisms
1. Define a differential medium and discuss its purpose.
2. Define a selective medium and describe its uses.
3. Why is MacConkey agar selective as well as differential?
4. Why is blood agar useful as a primary isolation medium?
5. What is the major difference between Modified Thayer-Martin (MTM) and chocolate agar? When would you use MTM rather than chocolate agar?
