Experiment 1: Transcription
Transcription is the production of RNA from DNA. It reads one strand of DNA, called the template, to create a new RNA strand. The nucleotides are added to the growing RNA strand in a 3′ to 5′ direction from the DNA strand.
Transcribe the following DNA sequence:Hint: By convention, a nucleic acid sequence is always written 5′ to 3′ unless otherwise notated.Hb A: AGT AAC GGC AGA CTT CTC CTC AGG AGT CAG GTG CAC CATTranslate your new RNA sequence using the genetic code. Remember that when determining your amino acid sequence, the RNA sequence is read from 5′ to 3′.Transcribe the following DNA sequence.Hb S: AGT AAC GGC AGA CTT CTC CAC AGG AGT CAG GTG CAC CATTranslate your Hb S transcript.