Solution-Plasminogen for directional subcloning

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Solution-Plasminogen for directional subcloning

Design PCR primers to amplify Kringles 2-4 of human plasminogen for directional subcloning into PBluescript. Show the exact sequence where the PCR product is directionally ligated (2 restriction enzymes) into the vector ( don’t show all the intervening DNA of the PCR product)

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